Hplc Profiling of Polyphenolic Compounds and Free Radical Assay of the Ethanolic Extract of Annona Reticulata Growing in Bangladesh

The present study investigates the HPLC profiling of phenolic compounds, and antioxidant activities of Annona reticulata. Through HPLC assay, high levels of rutin hydrate (603.88 mg/100 g extract) and moderate amount of catechin, epicatechin, and p-coumeric acid were determined (99.00, 78.19, 85.38 mg/100 g extract, respectively). Some amount of vanillic acid, ellagic acid, and quercetin were also detected (19.80, 31.56, 8.83 mg/100 g extract, respectively). Antioxidant activity tests such as, ABTS radical scavenging activity, reducing power, total antioxidant capacity, total phenolic and flavanoid content determination, were also performed. At 250 μg/ml, the percentage inhibition value of Annona reticulata for ABTS scavenging was 99.93 μg/ml, and the maximum absorbance for reducing power assay was 0.195. The total antioxidant capacity, and phenolic and flavonoid contents were calculated to be 419.20 mg of ascorbic acid/g, 120.07 mg/g of gallic acid, and 90.05 mg/g of quercetin equivalent, respectively. These results suggested that polyphenolic compounds in Annona reticulata might be responsible for their antioxidant activities.